The tube was inverted on a filter paper to drain the fluid. 50ul elution buffer was added to the tube and the contents mixed thoroughly using a vortex. Incubation followed for 15 minutes. The aim was to dissolve the DNA granules. 100ul HTR reagent was added to the contents and left to rest for 2 minutes to remove inhibitors. Centrifugation at 14000xg followed for 2 minutes, after which the cleared supernatant was transferred to a new 2ml centrifuge tube. An equal volume of Xp1 buffer was added to the contents and mixed thoroughly. The mixture was transferred to HiBind DNA mini column after its insertion into a 2ml collection tube. Centrifugation at 14000xg for a minute followed, after which the filtrate in the collection tube was discarded. 300ul XP1 buffer was added into the solution. Centrifugation followed for one minute at 10000xg. The mini pilaster was relocated to a new cylinder. Here, 700ul SPW wash buffer was added. The procedure preceded centrifugation. The filtrate was discarded and the washing procedure repeated.
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