The results of the investigation are laid out in a coherent sequence. The authors intend to establish the potential of gadonanotubes asÂ in vitroÂ tracers for magnetic resonance imaging as well as the effects of GNTs on living cells. The quantitative techniques as depicted by the results of the study are appropriate for this quest. For instance, it is expected that GNTs should be eliminated from living cells after fulfilling their role of enhancing MR imaging. A pulse-chase investigation is carried out by quantifying the concentration of Gd3+Â ions in labelled cells under varying conditions to determine the ability of GNTs to leave the cells (Tran et al. 2010). Similarly, the rate of proliferation of labelled cells is compared to the rate of propagation of unlabelled cells. A comparison of the propagation rate of labelled and unlabelled cells reveals that unlabelled cells multiply much faster than the labelled cells implying that Gd3+Â leads to the death of cells within the first 48 hours. These findings are significant in a clinical setting because they illustrate the expected outcomes if gadonanotubes are used to label stem cells. Conversely, in the evaluation of the self-renewal attributes and propagation dynamics of mesenchymal stem cells labelled with GNTs, the â€œcolony-forming unit fibroblast (CFU-F) and population doubling time (PDT) assaysâ€ of the labelled cells are carried out (Tran et al. 2010, p. 9489). These tests make use of averages (colony-forming units and time in hours) as well as standard deviations. A comparison of these parameters in labelled and unlabelled cells reveals the efficacy of GNT labelling of MSCs. Therefore, it can be deduced that the quantitative methods used are appropriate for the kind of information needed because the methods not only provide numerical data, but also correspond to the physiological processes that take place in the cells.
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