The methodology of the SDS-PAGE technique involves obtaining a backing plat

The methodology of the SDS-PAGE technique involves obtaining a backing plate, glass plate, a comb, and 2 spacers. All the equipments obtained were thoroughly washed with the Pyroneg detergent, making sure that nay dirt from the plates was removed. Thereafter, ethanol was used to wipe down the plates, and they were let to dry. Caster stand, gel caster, and locks were obtained. The preparing solutions for resolving and stacking gels for Tris-Glycine SDS PAGE were also obtained. For the 12 % resolving, 3000 µL with a concentration of 40% Acrylamide was required. In addition, 2600 µL 1.5M Tris with a PH of 8.8, and 4192 µL H2O was also required. To this was added 100 µL 10% SDS and 100 µL 10% APS and 8 µL TEMED.

For the 5% Stacker, 3000 µL 40% Acrylamide was required. In addition, 4192 µL H2O and 2600 µL 1.5M Tris with a PH of 8.8 was necessary. In addition, 100 µL 10% SDS and 100 µL 10% APS and 8 µL TEMEDwould also be required. The apparatus was then added. After the gel polymerises had been resolved, the stacking gel was poured. The combs had to be placed into the stacking gel prior to its setting.

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