The trichrome stain also highlights Mallory hyaline and megamitochondria in alcoholic liver disease

“The trichrome stain also highlights Mallory hyaline and megamitochondria in alcoholic liver disease” (Macsween, 2007, p. 130). This is required in revealing the architecture of the tissue and any other evidence for the formation of nodule observed in cirrhosis, a PERLS stain for deposits of iron, a Chromotrop-Anilinblue (CAB) stain, a Periodic Anti-Schiff (PAS) applicable in detecting the alpha1 – antitrypsin bodies observed in the genetic disorder alpha1- antitrypsin deficiency. Both Giemsa and H&E will be suitable for staining biopsies from the stomach (Odze and Goldblum, 2009, p. 123; Fudge, 2000, p. 233).

The former will reveal Helicobacter – a type of bacteria that is commonly causes stomach ulcers and gastritis (Gislason and Alpha Nutrition Health Education, 2003, p. 73; Turkington and Ashby, 2007, p. 141). The two commonly used special stains in this practical are the Congo red and the Masson’s trichrome. They will be used in staining some tissue sections and in revealing some important diagnostic features.

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