The application of the affinity column technique has had many challenges. The issues revolve around the specificity and sensitivity of the eluted fractions, which in most cases are proteins (Arakawa et al. 2007). Proteins are prone to degradation when subjected to physical and chemical processes. The challenge often lies in developing affinity column techniques that will allow the maximum collection of the target proteins with no or minimal effects (Mattiasson et al. 1999) Various studies have been conducted to establish how affinity chromatography can be improved for effective use in protein elution and quantification (Melander et al. 1999). The following is a review of various studies conducted to improve protein recovery.
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