Prior to the extraction process, SPW wash buffer was prepared. The preparation was carried out by diluting SPW with 100% ethanol. The buffer was stored under room temperature. The heat elution buffer was set to 700C. A bucket containing ice was prepared and the microfuge set at 40C. 500 mg glass beads were added to a 15ml centrifuge tube. A starting sample of 0.4g of marine soil was added to the centrifuge tube containing the glass beads. 1ml of SLX-Mlus buffer was added and the contents vortexed using a mixer mill for 4 minutes. The aim of this process was to lyse the samples. The glass pebbles lyse the cells by breaking them open, releasing their intracellular components (Zhou 2011). Vortexing agitates the glass pellets to enable them lyse the cells.
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