Pellets were resuspended in dH2O or 1xPBS by utilizing the available OD values, in accordance with the relationship provided below:
O.D. = 100x buffer
100ÂµL PBS= 1 O.D.
The most ideal amount of buffer was used to resuspend pellets, and vortex samples used to facilitate bacterial pellet break up. Samples were boiled for a period of approximately 5 minutes. Thereafter, the 6X SDS loading was added. Again the samples were boiled for a period of between 5 and 10 minutes. Approximately 20 Âµl samples were loaded onto the gel to facilitate the commencement of ++Q
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