The pH of buffers can be computed using the Henderson-Hasselbalch equation, which is written as pH = pKaÂ + log ([Aâ€“]/[HA]). In this equation, pKaÂ is the logarithm of the acidâ€™s KaÂ value, [Aâ€“] is the concentration of the conjugate base in moles and [HA] is the strength of the acid in moles (before the dissociation). The ability of a weak acid or base to act as a buffer can be determined experimentally by the addition of a strong acid or base and measuring the resultant pH.
This practical aimed at using the Henderson-Hasselbalch equation to calculate the changes in pH of acetate and ammonium buffers after the addition of small quantities of hydrochloric acid and sodium hydroxide. It also sought to compare the experimental and calculated pH values.
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