Prior to the preparation of the gel

Prior to the preparation of the gel, the casting tray was inserted into the electrophoresis chamber. The tray was put into the chamber with the orange gaskets against the side walls. The comb was placed into the first set of slots. The slots are located at the top of the tray. During these procedures, the researcher made sure that one can clearly read 1.5 on the left side of the comb when placed properly in the tray. 40 ml was the volume of agarose gel solution needed for the purposes of this procedure.

The right quantity of agarose to make a 0.8% solution was measured out and placed in a 125ml Erlenmeyer flask. During the measurement, it was determined that agarose gels are cast as a percent weight per volume solutions (%w/v). For example, 100 mls of a 2% (w/v) solution is 2g/100ml. An appropriate amount of 50X TAE was added into the flask so that the final concentration was 1X. 35ml dH2O was then added into the flask and the contents swirled gently. The flask was then placed in the microwave and heated for 1 minute. After heating, the flask was removed, swirled gently, and put back into the microwave for an additional 30 seconds.

Don't use plagiarized sources. Get Your Custom Essay on
Prior to the preparation of the gel
Just from $13/Page
Order Essay

After heating, the flask was allowed to cool for 2 minutes before its contents were poured into a 50ml graduated cylinder. The volume of the flask was brought to 40mls through the addition of dH2O. The gel solution was returned back into the flask. 1µl of ethidium bromide was added into the combination. The researcher took caution when handling ethidium bromide as it is a hazardous substance. For example, the substance is a known mutagen and a suspected carcinogen. As a result of this, gloves were worn when handling it. The gel solution was then poured into the casting tray and allowed to sit for 15 minutes for it to solidify. The gel turned opaque with a slight blue hue when it was ready.

Assignment Help Guys
Calculate your paper price
Pages (550 words)
Approximate price: -

Why Work with Us

Top Quality and Well-Researched Papers

We always make sure that writers follow all your instructions precisely. You can choose your academic level: high school, college/university or professional, and we will assign a writer who has a respective degree.

Professional and Experienced Academic Writers

We have a team of professional writers with experience in academic and business writing. Many are native speakers and able to perform any task for which you need help.

Free Unlimited Revisions

If you think we missed something, send your order for a free revision. You have 10 days to submit the order for review after you have received the final document. You can do this yourself after logging into your personal account or by contacting our support.

Prompt Delivery and 100% Money-Back-Guarantee

All papers are always delivered on time. In case we need more time to master your paper, we may contact you regarding the deadline extension. In case you cannot provide us with more time, a 100% refund is guaranteed.

Original & Confidential

We use several writing tools checks to ensure that all documents you receive are free from plagiarism. Our editors carefully review all quotations in the text. We also promise maximum confidentiality in all of our services.

24/7 Customer Support

Our support agents are available 24 hours a day 7 days a week and committed to providing you with the best customer experience. Get in touch whenever you need any assistance.

Try it now!

Calculate the price of your order

Total price:

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

No need to work on your paper at night. Sleep tight, we will cover your back. We offer all kinds of writing services.


Essay Writing Service

No matter what kind of academic paper you need and how urgent you need it, you are welcome to choose your academic level and the type of your paper at an affordable price. We take care of all your paper needs and give a 24/7 customer care support system.


Admission Essays & Business Writing Help

An admission essay is an essay or other written statement by a candidate, often a potential student enrolling in a college, university, or graduate school. You can be rest assurred that through our service we will write the best admission essay for you.


Editing Support

Our academic writers and editors make the necessary changes to your paper so that it is polished. We also format your document by correctly quoting the sources and creating reference lists in the formats APA, Harvard, MLA, Chicago / Turabian.


Revision Support

If you think your paper could be improved, you can request a review. In this case, your paper will be checked by the writer or assigned to an editor. You can use this option as many times as you see fit. This is free because we want you to be completely satisfied with the service offered.

error: Content is protected !!
Open chat
Need assignment help? You can contact our live agent via WhatsApp using +1 718 717 2861

Feel free to ask questions, clarifications, or discounts available when placing an order.