DNA isolation usually starts with tissue/cell breakdown (lyses) (Anderson 2).This essentially involves protein structure destruction and facilitation of nucleic acids release. The process is often performed in a salt solution, comprising of protein denaturing detergents or proteases (Anderson 2). Cell membranes are dissolved and the cells broken down. Enzymes e.g. those found in meat tenderizer and pineapple juice facilitate protein precipitation and free DNA into the resulting solution (Anderson 2).
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